ARG58745
anti-ZEB2 antibody
anti-ZEB2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ZEB2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Hm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ZEB2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 948-962 of Human ZEB2. (DMQQRRKYQRKQGFQ) |
| 偶联标记 | Un-conjugated |
| 別名 | Zinc finger E-box-binding homeobox 2; SIP-1; SIP1; SMADIP1; Smad-interacting protein 1; Zinc finger homeobox protein 1b; HSPC082; ZFHX1B |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O60315 Human Zinc finger E-box-binding homeobox 2 Swiss-port # Q9R0G7 Mouse Zinc finger E-box-binding homeobox 2 |
|---|---|
| 基因名称 | ZEB2 |
| 全名 | zinc finger E-box binding homeobox 2 |
| 背景介绍 | The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010] |
| 生物功能 | Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 136 kDa |
| 翻译后修饰 | Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG58745 anti-ZEB2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58745 anti-ZEB2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58745 anti-ZEB2 antibody WB image
Western blot: 1) 293T and 2) HeLa cell lysates stained with ARG58745 anti-ZEB2 antibody.
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ARG58745 anti-ZEB2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58745 anti-ZEB2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58745 anti-ZEB2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human glioma tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58745 anti-ZEB2 antibody at 1 µg/ml dilution, overnight at 4°C.
