ARG66302

anti-Vimentin antibody [SQab1859]

anti-Vimentin antibody [SQab1859] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Chicken,Dog,Goat,Hamster,Human,Monkey,Mouse,Pig,Rat,Xenopus,Zebrafish

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
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anti-Vimentin antibody [SQab1859] Mouse Monoclonal antibody [SQab1859] recognizes Vimentin ARG66302 Arigo biolaboratories
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anti-Vimentin antibody [SQab1859] is rated 5.0 out of 5 by 4.

anti-Vimentin antibody [SQab1859]

概述

产品描述 Mouse Monoclonal antibody [SQab1859] recognizes Vimentin
反应物种 Hu, Ms, Rat, Chk, Dog, Goat, Hm, Mk, Pig, Xenopus, Zfsh
应用 FACS, ICC/IF, IHC-Fr, IHC-P, WB
特异性 This antibody recognizes exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
宿主 Mouse
克隆 Monoclonal
克隆号 SQab1859
同位型 IgG1
靶点名称 Vimentin
抗原物种 Bovine
抗原 A cytoskeletal Vimentin extract of bovine lens. 
偶联标记 Un-conjugated
全名 vimentin
別名 Vimentin; CTRCT30; HEL113

应用说明

应用建议
应用 推荐稀释比
FACS1:100 - 1:200
ICC/IF1:50 - 1:250
IHC-Fr1:50 - 1:250
IHC-P1:50 - 1:250
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 54 kDa
实际分子量 ~57 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100522394 Pig VIM

GeneID: 22352 Mouse VIM

GeneID: 407747 Pig VIM

基因名称 VIM
基因全名 vimentin
背景介绍 Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
生物功能 Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
产品亮点 Related products:
Vimentin antibodies; Vimentin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
研究领域 Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
翻译后修饰 Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

检测图片 (6) Click the Picture to Zoom In

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of 22RV1 and DU145 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of COLO205 and SW620 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] IHC-Fr image

    Immunohistochemistry: Frozen section of swine colon stained with ARG66302 anti-Vimentin antibody [SQab1859] (green) at 1:200 dilution. DAPI (blue) staining for cell nuclei.

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:1000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:1000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] IHC-P image

    Immunohistochemistry: paraffin section of Human colon stained with ARG66302 anti-Vimentin antibody [SQab1859].

客户反馈

nuts_pic      Excellent

anti-Vimentin antibody [SQab1859]

Application:WB

Sample:COLO205 and SW620

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-Vimentin antibody [SQab1859]

Application:WB

Sample:22RV1 and DU145

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-Vimentin antibody [SQab1859]

Application:WB

Sample:HeLa and 293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-Vimentin antibody [SQab1859]

Application:WB

Sample:293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC