ARG65682

anti-Vimentin antibody

anti-Vimentin antibody for Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
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anti-Vimentin antibody

Mouse Monoclonal antibody recognizes Vimentin

ARG65682 Arigo biolaboratories
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anti-Vimentin antibody is rated 4.5 out of 5 by 2.

anti-Vimentin antibody

概述

产品描述

Mouse Monoclonal antibody recognizes Vimentin

反应物种 Hu, Ms, Rat
预测物种 Yeast
应用 WB
宿主 Mouse
克隆 Monoclonal
靶点名称 Vimentin
抗原物种 Human
抗原 Recombinant Protein of Human Vimentin (NP_003371.2).
偶联标记 Un-conjugated
全名 vimentin
別名 Vimentin; CTRCT30; HEL113

应用说明

应用建议
应用 推荐稀释比
WB1:1000 - 1:3000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 54 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 22352 Mouse VIM

GeneID: 7431 Human VIM

GeneID: 81818 Rat VIM

基因名称 VIM
基因全名 vimentin
背景介绍 Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
生物功能 Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
产品亮点 Related products:
Vimentin antibodies; Vimentin Duos / Panels;
Related news:
New antibody panels and duos for Tumor immune microenvironment
研究领域 Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
翻译后修饰 Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

检测图片 (1) Click the Picture to Zoom In

  • ARG65682 anti-Vimentin antibody WB image

    Western blot: 30 µg of 1) MDA-MB-231, and 2) MCF7 (negative control) cell lysates stained with ARG65682 anti-Vimentin antibody at 1:500 dilution.

客户反馈

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anti-Vimentin antibody

Application:WB

Sample:1) A549 (lung cancer), 2) H1299 (meta lung cancer)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-Vimentin antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC