ARG55540

anti-Vimentin antibody

anti-Vimentin antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Monkey

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
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anti-Vimentin antibody

Mouse Monoclonal antibody recognizes Vimentin

ARG55540 Arigo biolaboratories
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anti-Vimentin antibody

概述

产品描述

Mouse Monoclonal antibody recognizes Vimentin

反应物种 Hu, Mk
应用 ICC/IF, IP, WB
特异性 This antibody detects endogenous levels of Vimentin and does not cross-react with related proteins.
宿主 Mouse
克隆 Monoclonal
同位型 IgG2a
靶点名称 Vimentin
抗原物种 Human
抗原 Purified recombinant fragment of Human Vimentin protein (NP_003371.2).
偶联标记 Un-conjugated
全名 vimentin
別名 Vimentin; CTRCT30; HEL113

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:800
IPAssay-dependent
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 54 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol
抗菌剂 0.03% Proclin 300
稳定剂 50% Glycerol
浓度 1.64 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 7431 Human VIM

Swiss-port # P08670 Human Vimentin

基因名称 VIM
基因全名 vimentin
背景介绍 Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
生物功能 Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
产品亮点 Related products:
Vimentin antibodies; Vimentin Duos / Panels; Anti-Mouse IgG secondary antibodies;
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研究领域 Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
翻译后修饰 Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

检测图片 (6) Click the Picture to Zoom In

  • ARG55540 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55540 anti-Vimentin antibody (green) at 1:800 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG55540 anti-Vimentin antibody WB image

    Western blot: 20 µg of Colo205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG55540 anti-Vimentin antibody at 1:2000 dilution.

  • ARG55540 anti-Vimentin antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG55540 anti-Vimentin antibody.

  • ARG55540 anti-Vimentin antibody WB image

    Western blot: 20 µg of H1299 and A549 cell lysates stained with ARG55540 anti-Vimentin antibody at 1:1000 dilution.

  • ARG55540 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55540 anti-Vimentin antibody (green) at 1:800 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG55540 anti-Vimentin antibody WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG55540 anti-Vimentin antibody at 1:1000 dilution.

客户反馈

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anti-Vimentin antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:800

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

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anti-Vimentin antibody

Application:WB

Sample:Colo205, HCT116, HT29, SW480 and SW620

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-Vimentin antibody

Application:WB

Sample:H1299 and A549

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-Vimentin antibody

Application:WB

Sample:293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-Vimentin antibody

Application:WB

Sample:A: MCF-7 (breast cancer) (negative control), B: MDA-MB-231 (meta breast cancer)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC