ARG63632
anti-UBE2I / UBC9 antibody
anti-UBE2I / UBC9 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes UBE2I / UBC9 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2I / UBC9 |
| 抗原物种 | Human |
| 抗原 | SGIALSRLAQERK-C |
| 偶联标记 | Un-conjugated |
| 別名 | UBC9; Ubiquitin-protein ligase I; EC 6.3.2.-; SUMO-conjugating enzyme UBC9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; SUMO-protein ligase; p18; C358B7.1; P18; Ubiquitin carrier protein 9 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | Phosphorylation at Ser-71 significantly enhances SUMOylation activity. |
检测图片 (3) Click the Picture to Zoom In
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ARG63632 anti-UBE2I / UBC9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney. (Microwaved antigen retrieval with Tris/EDTA buffer pH 9.0) stained with ARG63632 anti-UBE2I / UBC9 antibody at 0.3 µg/ml dilution followed by HRP-staining.
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ARG63632 anti-UBE2I / UBC9 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63632 anti-UBE2I / UBC9 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63632 anti-UBE2I / UBC9 antibody WB image
Western blot: 35 µg of Rat ovary lysate (in RIPA buffer) stained with ARG63632 anti-UBE2I / UBC9 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
