ARG57704
anti-TRIM21 antibody
anti-TRIM21 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TRIM21 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM21 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human TRIM21. |
| 偶联标记 | Un-conjugated |
| 別名 | RNF81; Ro; EC 6.3.2.-; SSA1; SS-A; RO52; Ro/SSA; E3 ubiquitin-protein ligase TRIM21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sjoegren syndrome type A antigen; 52 kDa Ro protein; RING finger protein 81; SSA; Tripartite motif-containing protein 21 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | A549 | ||||||||||
| 实际分子量 | ~ 54 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P19474 Human E3 ubiquitin-protein ligase TRIM21 |
|---|---|
| 基因名称 | TRIM21 |
| 全名 | tripartite motif containing 21 |
| 背景介绍 | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. [UniProt] |
| 细胞定位 | Cytoplasm, Cytoplasmic vesicle, Nucleus, P-body, autophagosome. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG57704 anti-TRIM21 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG57704 anti-TRIM21 antibody at 1:100 dilution.
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ARG57704 anti-TRIM21 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human appendix tissue stained with ARG57704 anti-TRIM21 antibody at 1:100 dilution.
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ARG57704 anti-TRIM21 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG57704 anti-TRIM21 antibody at 1:1000 dilution.
