ARG56671

anti-TGF alpha antibody

anti-TGF alpha antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Hamster

概述

产品描述 Rabbit Polyclonal antibody recognizes TGF alpha
反应物种 Hu, Hm
应用 ELISA, IHC-P, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 TGF alpha
抗原物种 Human
抗原 E.coli derived Recombinant Human TGF-α.
(VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA)
偶联标记 Un-conjugated
別名 ETGF; EGF-like TGF; TFGA; Protransforming growth factor alpha; TGF-alpha; TGF type 1

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56781 as a detection antibody
IHC-P0.5 µg/ml
Neut2.5 - 3.7µg/ml (To yield [ND50] of the biological activity of Human TGF - α (0.5 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 7039 Human TGFA

Swiss-port # P01135 Human Protransforming growth factor alpha

基因名称 TGFA
全名 transforming growth factor, alpha
背景介绍 This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
生物功能 TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. [UniProt]
预测分子量 17 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG56671 anti-TGF alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56671 anti-TGF alpha antibody concentration is 0.5 µg/ml with a two-hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56671 anti-TGF alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56671 anti-TGF alpha antibody concentration is 0.5 µg/ml with a two-hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56671 anti-TGF alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56671 anti-TGF alpha antibody concentration is 0.5 µg/ml with a two-hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.