ARG10751
anti-TAF15 antibody [4D71]
anti-TAF15 antibody [4D71] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [4D71] recognizes TAF15 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4D71 |
| 同位型 | IgG1 |
| 靶点名称 | TAF15 |
| 抗原物种 | Human |
| 抗原 | Full length recombinant Human TAF15 expressed in and purified from E. coli. |
| 偶联标记 | Un-conjugated |
| 別名 | RBP56; TATA-binding protein-associated factor 2N; RNA-binding protein 56; TAF2N; II; Npl3; TAF; TAFII68; 68 kDa TATA-binding protein-associated factor |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q92804 Human TATA-binding protein-associated factor 2N |
|---|---|
| 基因名称 | TAF15 |
| 全名 | TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa |
| 背景介绍 | This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| 生物功能 | RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). [UniProt] |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. ADP-ribosylated during genotoxic stress. |
检测图片 (4) Click the Picture to Zoom In
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ARG10751 anti-TAF15 antibody [4D71] ICC/IF image
Immunocytochemistry: HeLa cell cultures stained with ARG10751 anti-TAF15 antibody [4D71] (green) and co-stained with chicken antibody to vimentin (red). ARG10751 anti-TAF15 antibody [4D71] reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.
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ARG10751 anti-TAF15 antibody [4D71] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10751 anti-TAF15 antibody [4D71] (red) at 1:500 dilution and costained with Rabbit pAb to GAPDH (green) at 1:1000 dilution. Hoechst (blue) for nuclear staining.
Clone 4D71 reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.
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ARG10751 anti-TAF15 antibody [4D71] WB image
Western blot: 20 µg of 1) HeLa, and 2) Hek293 cell lysates stained with ARG10751 anti-TAF15 antibody [4D71] at 1: 1000 dilution.
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ARG10751 anti-TAF15 antibody [4D71] WB image
Western blot: NIH/3T3 (cytosol), NIH/3T3 (nuclear), HeLa (cytosol) and HeLa (nuclear). The blots were stained with ARG10751 anti-TAF15 antibody [4D71] (green) at 1:1000 dilution.
