ARG54888

anti-Slug antibody

anti-Slug antibody for Western blot and Human

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
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publication_link 参考文献2

概述

产品描述 Rabbit Polyclonal antibody recognizes Slug
反应物种 Hu
预测物种 Ms, Rat, Bov
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Slug
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human Slug.
偶联标记 Un-conjugated
別名 Protein snail homolog 2; SNAIL2; WS2D; Neural crest transcription factor Slug; Zinc finger protein SNAI2; SLUG; SLUGH1

应用说明

应用建议
应用 推荐稀释比
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 A2058

属性

形式 Liquid
纯化 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液 PBS and 0.09% (W/V) Sodium azide
抗菌剂 0.09% (W/V) Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6591 Human SNAI2

Swiss-port # O43623 Human Zinc finger protein SNAI2

基因名称 SNAI2
全名 snail family zinc finger 2
背景介绍 This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]
生物功能 Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis. [UniProt]
细胞定位 Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions
研究领域 Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
预测分子量 30 kDa
翻译后修饰 GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.

检测图片 (1) Click the Picture to Zoom In

  • ARG54888 anti-Slug antibody WB image

    Western blot: 35 µg of A2058 cell lysate stained with ARG54888 anti-Slug antibody.

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anti-Slug antibody

Application:WB

Sample:A549 and H1299

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion

WB / Human

Ding-Ping Sun et al.
Hum Exp Toxicol.,  (2023)

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A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)

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