anti-E Cadherin antibody [SQab1717]

anti-E Cadherin antibody [SQab1717] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

EMT Study antibody; Epithelial Marker antibody
publication_link 参考文献4


产品描述 Recombinant Rabbit Monoclonal antibody [SQab1717] recognizes E Cadherin
反应物种 Hu
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1717
靶点名称 E Cadherin
抗原物种 Human
抗原 Synthetic peptide around aa. 600‐700 of E‐Cadherin.
偶联标记 Un-conjugated
別名 Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324


应用 推荐稀释比
WB1:1000  ‐ 1:5000
应用说明 IHC: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 999 Human CDH1

Swiss-port # P12830 Human Cadherin-1

基因名称 CDH1
全名 cadherin 1, type 1
背景介绍 E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
生物功能 Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30320 EMT Marker Antibody Panel
Related products:
E Cadherin antibodies; E Cadherin ELISA Kits; E Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
New EMT antibody panel is released
研究领域 EMT Study antibody; Epithelial Marker antibody
预测分子量 97 kDa
翻译后修饰 During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
N-glycosylation at Asn-637 is essential for expression, folding and trafficking.
Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

检测图片 (3) Click the Picture to Zoom In

  • ARG66195 anti-E Cadherin antibody [SQab1717] WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:2000 dilution.

  • ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human skin tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human gastric adenocarcinoma tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0


nuts_pic      Excellent

anti-E Cadherin antibody [SQab1717]



Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC


Coordination of LMO7 with FAK Signaling Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure

ICC/IF / Rat

Yen-Yi Zhen et al.
cells,  (2022)




Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)




A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)




Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)