ARG41968
anti-SMN / Gemin1 antibody [2B10]
anti-SMN / Gemin1 antibody [2B10] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [2B10] recognizes SMN / Gemin1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2B10 |
| 同位型 | IgG1 |
| 靶点名称 | SMN / Gemin1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 22-52 of Human SMN / Gemin1. (RRGTGQSDDSDIWDDTALIKAYDKAVASFKH) |
| 偶联标记 | Un-conjugated |
| 別名 | SMA3; SMA2; SMA1; Component of gems 1; SMNT; SMA4; GEMIN1; Survival motor neuron protein; BCD541; SMN; SMA@; T-BCD541; TDRD16A; SMA; Gemin-1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SMN1 |
| 全名 | survival of motor neuron 1, telomeric |
| 背景介绍 | This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. However, mutations in this gene, the telomeric copy, are associated with spinal muscular atrophy; mutations in the centromeric copy do not lead to disease. The centromeric copy may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7, which is thought to be an exon splice enhancer. Note that the nine exons of both the telomeric and centromeric copies are designated historically as exon 1, 2a, 2b, and 3-8. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein. Multiple transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2014] |
| 生物功能 | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). [UniProt] |
| 细胞定位 | Nucleus, gem, Cajal body. Cytoplasm. Cytoplasmic granule. Perikaryon. Cell projection. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with actin and at the Z-line of skeletal muscle. Under stress conditions colocalizes with RPP20/POP7 in punctuated cytoplasmic granules. Colocalized and redistributed with ZPR1 from the cytoplasm to nuclear gems (Gemini of coiled bodies) and Cajal bodies. Colocalizes with FMR1 in cytoplasmic granules in the soma and neurite cell processes. [UniProt] |
| 预测分子量 | 32 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG41968 anti-SMN / Gemin1 antibody [2B10] ICC image
Immunocytochemistry: A431 cells were blocked with 10% goat serum and then stained with ARG41968 anti-SMN / Gemin1 antibody [2B10] at 1 µg/ml dilution, overnight at 4°C.
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ARG41968 anti-SMN / Gemin1 antibody [2B10] IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41968 anti-SMN / Gemin1 antibody [2B10] at 1 µg/ml dilution, overnight at 4°C.
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ARG41968 anti-SMN / Gemin1 antibody [2B10] WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, Human placenta, SW620, PANC-1, HepG2, A549, RH35 and HEPA1-6 whole cell lysates stained with ARG41968 anti-SMN / Gemin1 antibody [2B10] at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41968 anti-SMN / Gemin1 antibody [2B10] IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41968 anti-SMN / Gemin1 antibody [2B10] at 1 µg/ml dilution, overnight at 4°C.
