ARG63095
anti-SIT antibody [SIT-01]
anti-SIT antibody [SIT-01] for Flow cytometry,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [SIT-01] recognizes SIT |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IP, WB |
| 特异性 | The clone SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SIT-01 |
| 同位型 | IgG1 |
| 靶点名称 | SIT |
| 抗原物种 | Human |
| 抗原 | Bacterially produced recombinant intracellular fragment of human SIT. |
| 偶联标记 | Un-conjugated |
| 別名 | gp30/40; Signaling threshold-regulating transmembrane adapter 1; SIT; SHP2-interacting transmembrane adapter protein; SIT-R; Suppression-inducing transmembrane adapter 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Staining technique: Intracellular staining. WB: WB analysis demonstrated that SIT is expressed as an ~ 40 kDa protein that is reduced to ~ 20 kDa by endoglycosidase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9Y3P8 Human Signaling threshold-regulating transmembrane adapter 1 |
|---|---|
| 基因名称 | SIT1 |
| 全名 | signaling threshold regulating transmembrane adaptor 1 |
| 背景介绍 | SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors. |
| 生物功能 | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK. |
检测图片 (1) Click the Picture to Zoom In
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ARG63095 anti-SIT antibody [SIT-01] WB image
Western blot: Jurkat cell lysate (1% lauryl maltoside) (non-reduced sample) stained with ARG63095 anti-SIT antibody [SIT-01].
