ARG43063
anti-SETD1A / SET1A antibody
anti-SETD1A / SET1A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SETD1A / SET1A |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SETD1A / SET1A |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to D8-K153 of Human SETD1A / SET1A. |
| 偶联标记 | Un-conjugated |
| 別名 | KMT2F; Set1/Ash2 histone methyltransferase complex subunit SET1; Histone-lysine N-methyltransferase SETD1A; hSET1A; Lysine N-methyltransferase 2F; EC 2.1.1.43; Set1; Set1A; SET domain-containing protein 1A |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O15047 Human Histone-lysine N-methyltransferase SETD1A |
|---|---|
| 基因名称 | SETD1A |
| 全名 | SET domain containing 1A |
| 背景介绍 | The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. [provided by RefSeq, Dec 2016] |
| 生物功能 | Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. [UniProt] |
| 细胞定位 | Nucleus speckle. Chromosome. Note=Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes. [UniProt] |
| 预测分子量 | 186 kDa |
检测图片 (6) Click the Picture to Zoom In
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ARG43063 anti-SETD1A / SET1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43063 anti-SETD1A / SET1A antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43063 anti-SETD1A / SET1A antibody WB image
Western blot: 50 µg of SW620 and U87 whole cell lysates stained with ARG43063 anti-SETD1A / SET1A antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43063 anti-SETD1A / SET1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43063 anti-SETD1A / SET1A antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43063 anti-SETD1A / SET1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43063 anti-SETD1A / SET1A antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43063 anti-SETD1A / SET1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43063 anti-SETD1A / SET1A antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43063 anti-SETD1A / SET1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43063 anti-SETD1A / SET1A antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
