ARG11060
anti-SCGN / Secretagogin antibody
anti-SCGN / Secretagogin antibody for IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow
概述
| 产品描述 | Chicken Polyclonal antibody recognizes SCGN / Secretagogin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow |
| 应用 | IHC-Fr, WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | SCGN / Secretagogin |
| 抗原物种 | Human |
| 抗原 | Full-length recombinant Human Secretagogin protein. |
| 偶联标记 | Un-conjugated |
| 別名 | SEGN; DJ501N12.8; setagin; SECRET; CALBL; Secretagogin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 27 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SCGN |
| 全名 | secretagogin, EF-hand calcium binding protein |
| 背景介绍 | The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008] |
| 预测分子量 | 32 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG11060 anti-SCGN / Secretagogin antibody IHC-Fr image
Immunohistochemistry: Mouse brain olfactory bulb section stained with ARG11060 anti-SCGN / Secretagogin antibody (red) at 1:1000 dilution, and costained with mouse mAb to calretinin (green) at 1:500 dilution. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.
Secretagogin is highly expressed in the mitral and granular cell layers, while calretinin is in the glomerular layer. Cells that express secretagogin or calretinin only are red or green respectively. A number of cells containing both proteins appears orange-yellow. Mouse select image at left for larger view.
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ARG11060 anti-SCGN / Secretagogin antibody WB image
Western blot: Mouse olfactory bulb, Rat cerebellum and Cow cerebellum lysates stained with ARG11060 anti-SCGN / Secretagogin antibody at 1:1000 dilution.
