ARG10739
anti-SCGN / Secretagogin antibody
anti-SCGN / Secretagogin antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SCGN / Secretagogin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SCGN / Secretagogin |
| 抗原物种 | Human |
| 抗原 | Full-length recombinant Human secretagogin protein. |
| 偶联标记 | Un-conjugated |
| 別名 | SEGN; DJ501N12.8; setagin; SECRET; CALBL; Secretagogin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SCGN |
| 全名 | secretagogin, EF-hand calcium binding protein |
| 背景介绍 | The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008] |
| 预测分子量 | 32 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG10739 anti-SCGN / Secretagogin antibody IHC image
IHC: Adult Mouse olfactory bulb floating section stained with ARG10739 anti-SCGN / Secretagogin antibody at 1:1000 (red) and co-stained with our chicken polyclonal antibody to calretinin at 1:1000 (green). Secretagogin is predominantly expressed in cells in external plexiform lay (EPL) and granular cell lay (GCL) in olfactory bulb. Secretagogin coexists with calretinin in some cells in GCL, so these cells appear yellow. Secretagogin is found in both cell body and processes. Blue is a DNA stain.
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ARG10739 anti-SCGN / Secretagogin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain cerebellum stained with ARG10739 anti-SCGN / Secretagogin antibody (green) at 1:1000 dilution and costained with Mouse mAb to calbindin (red) at 1:1000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
The SCGN antibody stains granule cells and interneurons while calbindin antibody reveals protein expressed in dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum.
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ARG10739 anti-SCGN / Secretagogin antibody WB image
Western blot: Rat pancreas lysate stained with ARG10739 anti-SCGN / Secretagogin antibody at 1:1000 dilution.
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ARG10739 anti-SCGN / Secretagogin antibody WB image
Western blot: Rat pancreas, Rat olfactory bulb and Mouse olfactory bulb lysates stained with ARG10739 anti-SCGN / Secretagogin antibody (green) at 1:1000 dilution.
