ARG43282

anti-SARS-CoV-2 Spike protein (S2) antibody

Datasheet

订购信息

包装

价格 (USD)

100 μg

599

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Rabbit Polyclonal antibody recognizes SARS-CoV-2 Spike protein (S2)
反应物种	Virus
应用	IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	SARS-CoV-2 Spike protein (S2)
抗原物种	Virus
抗原	A 16-amino acid synthetic peptide within aa. 1130-1180 of SARS-CoV-2 Spike protein.
偶联标记	Un-conjugated

应用说明

应用建议

应用	推荐稀释比
IHC-P	0.1 µg/ml
WB	1 µg/ml

应用说明

IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS and 0.02% Sodium azide.
抗菌剂	0.02% Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
生物功能	mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [UniProt]

背景介绍

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

生物功能

mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [UniProt]

检测图片 (3) Click the Picture to Zoom In

ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody at 0.1 µg/ml dilution, overnight at 4°C.

Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung (left), but not in non-COVID-19 patient lung (right).
ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody WB image

Western blot: 30 ng of SARS-CoV-2 Spike S2 recombinant protein stained with ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody at 1 µg/ml dilution, 1 hour incubation at RT in 5% NFDM/TBST.
ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody WB image

Western blot: Spike transfected 293 cells. 10 µg of cell lysate stained with ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody at 1 µg/ml dilution, 1 hour incubation at RT in 5% NFDM/TBST.