ARG66073

anti-Resistin antibody

anti-Resistin antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Goat Polyclonal antibody recognizes Resistin
反应物种 Hu, Ms
应用 ELISA, IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Resistin
抗原物种 Human
抗原 E. coli derived recombinant Human Resistin.
(SSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP)
偶联标记 Un-conjugated
別名 C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; XCP1; Resistin; Adipose tissue-specific secretory factor; FIZZ3; RETN1; ADSF; Cysteine-rich secreted protein FIZZ3; RSTN

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66074 as a detection antibody
IHC-P1 µg/ml
WB0.1 - 0.2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 56729 Human RETN

GeneID: 57264 Mouse RETN

Swiss-port # Q99P87 Mouse Resistin

Swiss-port # Q9HD89 Human Resistin

基因名称 RETN
全名 resistin
背景介绍 This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]
生物功能 Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. [UniProt]
预测分子量 11 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG66073 anti-Resistin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal placenta. The recommended ARG66073 anti-Resistin antibody concentration is 1.0 µg/ml with a two hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG66073 anti-Resistin antibody WB image

    Western blot: 250 - 0.24 ng of Human Resistin stained with ARG66073 anti-Resistin antibody, under non-reducing conditions.

  • ARG66073 anti-Resistin antibody standard curve image

    Sandwich ELISA: ARG66073 anti-Resistin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66074 anti-Resistin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66073 anti-Resistin antibody WB image

    Western blot: 250 - 0.24 ng of Human Resistin stained with ARG66073 anti-Resistin antibody, under reducing conditions.