anti-Rb1 / Retinoblastoma protein antibody

anti-Rb1 / Retinoblastoma protein antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
publication_link 参考文献2


产品描述 Rabbit Polyclonal antibody recognizes Rb1 / Retinoblastoma protein
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Rb1 / Retinoblastoma protein
抗原物种 Human
抗原 Peptide sequence around aa. 805~809 (Y-I-S-P-L) derived from Human Rb1 / Retinoblastoma.
偶联标记 Un-conjugated
別名 Rb; PPP1R130; pRb; pp110; RB; Retinoblastoma-associated protein; p105-Rb; OSRC


应用 推荐稀释比
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 19645 Mouse RB1

GeneID: 24708 Rat RB1

GeneID: 5925 Human RB1

基因名称 RB1
全名 retinoblastoma 1
背景介绍 The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
生物功能 Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30046 Phospho RB Antibody Duo (Total, pS807)
Related products:
Retinoblastoma protein antibodies; Retinoblastoma protein Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Senescence Marker Antibody Panel is launched
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
预测分子量 106 kDa
翻译后修饰 Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).
N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

检测图片 (2) Click the Picture to Zoom In

  • ARG51103 anti-Rb1 / Retinoblastoma protein antibody WB image

    Western blot: 20 µg of K562 cell lysate stained with ARG51103 anti-Rb1 / Retinoblastoma protein antibody at 1:500 dilution. Positive control: Tubulins were stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:4000 dilution.

  • ARG51103 anti-Rb1 / Retinoblastoma protein antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded human breast carcinoma tissue stained with ARG51103 anti-Rb1 / Retinoblastoma protein antibody (left) or the same antibody preincubated with blocking peptide (right).


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anti-Rb1 / Retinoblastoma protein antibody


Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC


Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)




Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.

WB / Human

Yang Yan et al.
Cancer Cell Int.,  (2020)