ARG58296

anti-RUNX1T1 / ETO antibody

anti-RUNX1T1 / ETO antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes RUNX1T1 / ETO
反应物种 Hu, Ms
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RUNX1T1 / ETO
抗原物种 Human
抗原 E. coli-derived Human RUNX1T1/ETO recombinant protein (Position: T335-D510).
偶联标记 Un-conjugated
別名 AML1T1; MTG8; CBFA2T1; ZMYND2; Protein MTG8; Protein CBFA2T1; Cyclin-D-related protein; Eight twenty one protein; CDR; AML1-MTG8; Protein ETO; Zinc finger MYND domain-containing protein 2; ETO

应用说明

应用建议
应用 推荐稀释比
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12395 Mouse RUNX1T1

GeneID: 862 Human RUNX1T1

Swiss-port # Q06455 Human Protein CBFA2T1

Swiss-port # Q61909 Mouse Protein CBFA2T1

基因名称 RUNX1T1
全名 runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
背景介绍 This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
生物功能 Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 68 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG58296 anti-RUNX1T1 / ETO antibody ICC/IF image

    Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG58296 anti-RUNX1T1 / ETO antibody (green) at 2 µg/ml dilution, overnight at 4°C.

  • ARG58296 anti-RUNX1T1 / ETO antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58296 anti-RUNX1T1 / ETO antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58296 anti-RUNX1T1 / ETO antibody WB image

    Western blot: 50 µg of Human placenta and Mouse testis lysates stained with ARG58296 anti-RUNX1T1 / ETO antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.

  • ARG58296 anti-RUNX1T1 / ETO antibody FACS image

    Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG58296 anti-RUNX1T1 / ETO antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG58296 anti-RUNX1T1 / ETO antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58296 anti-RUNX1T1 / ETO antibody at 1 µg/ml dilution, overnight at 4°C.