ARG54926

anti-RIPK3 / RIP3 antibody

anti-RIPK3 / RIP3 antibody for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody
publication_link 参考文献2

概述

产品描述 Rabbit Polyclonal antibody recognizes RIPK3 / RIP3
反应物种 Hu, Ms, Rat
应用 ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RIPK3 / RIP3
抗原物种 Mouse
抗原 A 14-amino acid peptide within the last 50 amino acids of murine RIPK3 / RIP3.
偶联标记 Un-conjugated
別名 Receptor-interacting serine/threonine-protein kinase 3; Receptor-interacting protein 3; RIP-3; RIP3; RIP-like protein kinase 3; EC 2.7.11.1

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
ICC/IF20 μg/ml
IHC-FrAssay-dependent
IHC-P5 μg/ml
IP20 μg/ml
WB0.1 - 0.5 μg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 A431 and C2C12
实际分子量 ~ 57 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11035 Human RIPK3

GeneID: 246240 Rat RIPK3

GeneID: 56532 Mouse RIPK3

基因名称 Ripk3
全名 receptor-interacting serine-threonine kinase 3
背景介绍 RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
生物功能 Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30344 Necrosome Antibody Panel
Related products:
RIPK3 antibodies; RIPK3 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
A non-autophatic role of Atg9a in necrosis and developmental bone formation
RIP1 activation and pathogenesis of NASH
Ripoptosome & Necrosome antibody panels are launched
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody
预测分子量 57 kDa
翻译后修饰 RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3. Phosphorylation at Ser-227 is required for binding MLKL.
Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B.

检测图片 (5) Click the Picture to Zoom In

  • ARG54926 anti-RIPK3 / RIP3 antibody ICC/IF image

    Immunofluorescence: Rat kidney cells stained with ARG54926 anti-RIPK3 / RIP3 antibody at 20 µg/ml dilution.

  • ARG54926 anti-RIPK3 / RIP3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG54926 anti-RIPK3 / RIP3 antibody at 2.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.

  • ARG54926 anti-RIPK3 / RIP3 antibody WB image

    Western blot: 15 µg of A431 cell lysate stained with ARG54926 anti-RIPK3 / RIP3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

  • ARG54926 anti-RIPK3 / RIP3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG54926 anti-RIPK3 / RIP3 antibody at 5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.

  • ARG54926 anti-RIPK3 / RIP3 antibody WB image

    Western blot: 15 µg of C2C12 cell lysate stained with ARG54926 anti-RIPK3 / RIP3 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

文献引用

PARP inhibitor rescues hearing and hair cell impairment in Cx26-null mice

IHC-Fr, WB / Mouse

Xiaohui Wang et al.
View.,  (2023)

publication_link

 

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RIP3/MLKL pathway-regulated necroptosis: A new mechanism of paclitaxel-induced peripheral neuropathy

WB / Rat

Dongyang Ma et al.
J Biochem Mol Toxicol.,  (2021)

publication_link

 

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