ARG55495
anti-Pyruvate Carboxylase antibody
anti-Pyruvate Carboxylase antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Pyruvate Carboxylase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Pyruvate Carboxylase |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human PC |
| 偶联标记 | Un-conjugated |
| 別名 | PCB; Pyruvic carboxylase; EC 6.4.1.1; Pyruvate carboxylase, mitochondrial |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A549 | ||||||||
| 实际分子量 | ~ 129 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P11498 Human Pyruvate carboxylase, mitochondrial |
|---|---|
| 基因名称 | PC |
| 全名 | pyruvate carboxylase |
| 背景介绍 | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. [UniProt] |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 130 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55495 anti-Pyruvate Carboxylase antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG55495 anti-Pyruvate Carboxylase antibody.
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ARG55495 anti-Pyruvate Carboxylase antibody WB image
Western blot: A549 cell lysate stained with ARG55495 anti-Pyruvate Carboxylase antibody.
