ARG43992
anti-Proteasome 20S LMP2 antibody
anti-Proteasome 20S LMP2 antibody for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Proteasome 20S LMP2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Proteasome 20S LMP2 |
| 抗原物种 | Human |
| 抗原 | Human Proteasome 20S LMP2 recombinant protein |
| 表达系统 | E.coli |
| 偶联标记 | Un-conjugated |
| Protein Full name | Proteasome subunit beta type-9 |
| 別名 | PSMB9; Proteasome 20S Subunit Beta 9; RING12; PSMB6i; Beta1i; LMP2 ; Multicatalytic Endopeptidase Complex Chain 7; Really Interesting New Gene 12 Protein; Large Multifunctional Peptidase 2; Proteasome Subunit Beta Type-9; Low Molecular Mass Protein 2; Proteasome Subunit Beta-1i; Proteasome Subunit Beta 9; Proteasome Chain 7; Macropain Chain 7; EC 3.4.25.1; Proteasome (Prosome, Macropain) Subunit, Beta Type, 9 ; Proteasome Catalytic Subunit 1i; Proteasome Subunit Beta 6i; Proteasome Subunit Beta1i; Proteasome-Related Gene; Proteasome Subunit Β1i; PRAAS3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified with Immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PSMB9 |
| 全名 | Proteasome 20S Subunit Beta 9 |
| 背景介绍 | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. |
| 生物功能 | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. |
| 细胞定位 | Cytoplasm, Nucleus, Proteasome |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Acetylation, Zymogen |
检测图片 (5) Click the Picture to Zoom In
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ARG43992 anti-Proteasome 20S LMP2 antibody IHC-P image
Immunohistochemistry: Humanrectum adenocarcinoma stained with ARG43992 anti-Proteasome 20S LMP2 antibody at 2 μg/ml dilution.
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ARG43992 anti-Proteasome 20S LMP2 antibody WB image
Western blot:Jurkat and THP-1 stained with ARG43992 anti-Proteasome 20S LMP2 antibody at 0.5 μg/mL dilution.
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ARG43992 anti-Proteasome 20S LMP2 antibody FACS image
Flow Cytometry: JK cells stained withARG43992 anti-Proteasome 20S LMP2 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43992 anti-Proteasome 20S LMP2 antibody WB image
Western blot: Rat thymus ARG43992 anti-Proteasome 20S LMP2 antibody at 0.5 μg/mL dilution.
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ARG43992 anti-Proteasome 20S LMP2 antibody WB image
Western blot: Mouse thymus ARG43992 anti-Proteasome 20S LMP2 antibody at 0.5 μg/mL dilution.
