ARG56710

anti-PLGF1 antibody

anti-PLGF1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes PLGF1
反应物种 Hu
应用 ELISA, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PLGF1
抗原物种 Human
抗原 E.coli derived Recombinant Human PlGF-1.
(MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR)
偶联标记 Un-conjugated
別名 PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56819 as a detection antibody
IHC-P2.0 µg/ml
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5228 Human PGF

Swiss-port # P49763 Human Placenta growth factor

基因名称 PGF
全名 placental growth factor
背景介绍 This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]
生物功能 Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt]
产品亮点 Related products:
PLGF1 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
The role of HDGF in tumor angiogenesis
预测分子量 25 kDa
翻译后修饰 N-glycosylated.

检测图片 (5) Click the Picture to Zoom In

  • ARG56710 anti-PLGF1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56710 anti-PLGF1 antibody concentration is 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56710 anti-PLGF1 antibody WB image

    Western blot: 250 - 0.24 ng of Human PlGF stained with ARG56710 anti-PLGF1 antibody, under reducing conditions.

  • ARG56710 anti-PLGF1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56710 anti-PLGF1 antibody concentration is 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56710 anti-PLGF1 antibody WB image

    Western blot: 250 - 0.24 ng of Human PlGF stained with ARG56710 anti-PLGF1 antibody, under non-reducing conditions.

  • ARG56710 anti-PLGF1 antibody standard curve image

    Sandwich ELISA: ARG56710 anti-PLGF1 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56819 anti-PLGF1 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.