ARG58140
anti-PLC gamma 2 antibody
anti-PLC gamma 2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PLC gamma 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLC gamma 2 |
| 抗原物种 | Human |
| 抗原 | Partial recombinant protein corresponding to aa. 1201-1258 of Human PLC gamma 2. |
| 偶联标记 | Un-conjugated |
| 別名 | Phospholipase C-gamma-2; PLC-IV; Phosphoinositide phospholipase C-gamma-2; PLC-gamma-2; Phospholipase C-IV; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; FCAS3; APLAID; EC 3.1.4.11 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2% Trehalose. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PLCG2 |
| 全名 | phospholipase C, gamma 2 (phosphatidylinositol-specific) |
| 背景介绍 | The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014] |
| 生物功能 | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. [UniProt] |
| 细胞定位 | Cytoplasmic, cell membrane. [UniProt] |
| 预测分子量 | 148 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG58140 anti-PLC gamma 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG58140 anti-PLC gamma 2 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
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ARG58140 anti-PLC gamma 2 antibody WB image
Western blot: Human placenta and Mouse spleen lysate stained with ARG58140 anti-PLC gamma 2 antibody at 0.5 µg/ml dilution.
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ARG58140 anti-PLC gamma 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human intestinal cancer tissue stained with ARG58140 anti-PLC gamma 2 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
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ARG58140 anti-PLC gamma 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse spleen tissue stained with ARG58140 anti-PLC gamma 2 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
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ARG58140 anti-PLC gamma 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat spleen tissue stained with ARG58140 anti-PLC gamma 2 antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
