ARG45487

anti-PKLR antibody

anti-PKLR antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes PKLR
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PKLR
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminal region of human PKLR.
偶联标记 Un-conjugated
別名 PKR; PKRL; PK1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Pyruvate kinase PKLR; Pyruvate kinase 1; EC 2.7.1.40; RPK; Red cell/liver pyruvate kinase; PKL

应用说明

应用建议
应用 推荐稀释比
IHC-P0.5-1 μg/ml
WB0.1-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 62 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 24651 Rat PKLR

GeneID: 5313 Human PKLR

Swiss-port # P12928 Rat Pyruvate kinase PKLR

Swiss-port # P30613 Human Pyruvate kinase PKLR

基因名称 PKLR
全名 pyruvate kinase, liver and RBC
背景介绍 The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能 Plays a key role in glycolysis. [UniProt]
细胞定位 Cytoplasm; Cytosol. [UniProt]
预测分子量 62 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45487 anti-PKLR antibody IHC-P image

    Immunohistochemistry: Human intestinal cancer stained with ARG45487 anti-PKLR antibody at 1 μg/ml dilution.

  • ARG45487 anti-PKLR antibody WB image

    Western blot: HepG2 stained with ARG45487 anti-PKLR antibody at 0.5 μg/ml dilution.

  • ARG45487 anti-PKLR antibody WB image

    Western blot: Rat liver stained with ARG45487 anti-PKLR antibody at 0.5 μg/ml dilution.

  • ARG45487 anti-PKLR antibody WB image

    Western blot: Mouse liver stained with ARG45487 anti-PKLR antibody at 0.5 μg/ml dilution.