ARG63744
anti-PAI1 / SERPINE1 antibody
anti-PAI1 / SERPINE1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PAI1 / SERPINE1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog |
| 应用 | ELISA, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PAI1 / SERPINE1 |
| 抗原物种 | Human |
| 抗原 | C-GFKIDDKGMAPALRH |
| 偶联标记 | Un-conjugated |
| 別名 | Serpin E1; PLANH1; Endothelial plasminogen activator inhibitor; Plasminogen activator inhibitor 1; PAI; PAI1; PAI-1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond. |
检测图片 (3) Click the Picture to Zoom In
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ARG63744 anti-PAI1 / SERPINE1 antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG63744 anti-PAI1 / SERPINE1 antibody at 0.3 µg/ml dilution.
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ARG63744 anti-PAI1 / SERPINE1 antibody WB image
Western Blot: A549 (A), HepG2 (B) and HeLa (C) lysates (35 µg protein in RIPA buffer) stained with ARG63744 anti-PAI1 / SERPINE1 antibody at 0.1 µg/ml dilution.
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ARG63744 anti-PAI1 / SERPINE1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Placenta. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63744 anti-PAI1 / SERPINE1 antibody at 4 µg/ml dilution followed by HRP-staining.
