ARG54605

anti-Osteocalcin antibody [OC4-30]

anti-Osteocalcin antibody [OC4-30] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Goat,Pig,Rabbit,Sheep

Developmental Biology antibody; Signaling Transduction antibody
publication_link 参考文献8

概述

产品描述

Mouse Monoclonal antibody [OC4-30] recognizes Osteocalcin

反应物种 Hu, Ms, Rat, Bov, Chk, Dog, Goat, Pig, Rb, Sheep
应用 ELISA, ICC/IF, IHC-Fr, IHC-P, WB
特异性 Includes the γ-carboxylated residue at position 17
宿主 Mouse
克隆 Monoclonal
克隆号 OC4-30
同位型 IgG2a
靶点名称 Osteocalcin
抗原物种 Bovine
抗原 Bovine osteocalcin
偶联标记 Un-conjugated
別名 OCN; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; OC; Bone Gla protein; BGP

应用说明

应用说明 Sandwich ELISA: 1 - 10 ug/ml.
Western blot: 1 - 10 ug/ml, reducing or non-reducing conditions.
Immunohistochemistry: 1 - 10 ug/ml, paraffin-embedded or frozen tissue sections. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 10 mM PBS (pH 7.4) and 1% BSA
稳定剂 1% BSA
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12096 Mouse BGLAP

GeneID: 12097 Mouse BGLAP2

GeneID: 25295 Rat BGLAP

基因名称 BGLAP
全名 bone gamma-carboxyglutamate (gla) protein
背景介绍 This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
生物功能 Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. [UniProt]
研究领域 Developmental Biology antibody; Signaling Transduction antibody
预测分子量 11 kDa
翻译后修饰 Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

检测图片 (2) Click the Picture to Zoom In

  • ARG54605 anti-Osteocalcin antibody [OC4-30] IHC-P image

    Immunohistochemistry: Parrafin-embedded chondroma tissue stained with ARG54605 anti-Osteocalcin antibody [OC4-30] at 1:200 (10 µg/ml) dilution, RT for 60 min. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.

  • ARG54605 anti-Osteocalcin antibody [OC4-30] IHC-P image

    Immunohistochemistry: Parrafin-embedded synovial sarcoma tissue stained with ARG54605 anti-Osteocalcin antibody [OC4-30] at 1:200 (10 µg/ml) dilution, RT for 60 min. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.

文献引用

Liraglutide in Combination with Insulin Has a Superior Therapeutic Effect to Either Alone on Fracture Healing in Diabetic Rats

WB / IHC-P / Rats

Hao Liu et al.
Diabetes Metab Syndr Obes.,  (2023)

publication_link

 

hr_line

Ablation of Discoidin Domain Receptor 1 Provokes an Osteopenic Phenotype by Regulating Osteoblast/Osteocyte Autophagy and Apoptosis

IHC-P / Mouse

Hsin-Chiao Chou et al.
Biomedicines,  (2022)

publication_link

 

hr_line

Inhibiting PHD2 in human periodontal ligament cells via lentiviral vector-mediated RNA interference facilitates cell osteogenic differentiation and periodontal repair

WB / Human

Di Cui et al.
J Leukoc Biol.,  (2021)

publication_link

 

hr_line

Simvastatin stimulates osteogenic differentiation of bone marrow mesenchymal stem cells.

ICC/IF / Mouse

Zhibin Guo et al.
Chinese Journal of Tissue Engineering Research.,  (2020)

publication_link

 

hr_line

Peripheral sensory nerves regulate orthodontic tooth movement-induced bone formation via Wnt5a signaling pathway.

IHC-P / Rat

Lu Yeming et al.
新医学,  (2019)

publication_link

 

hr_line

Identification of Gli1-interacting proteins during simvastatin-stimulated osteogenic differentiation of bone marrow mesenchymal stem cells.

WB, ICC/IF / Rat

Chi Bojing et al.
J Cell Biochem.,  (2019)

publication_link

 

hr_line

Stiffness Modification of Photopolymerizable Gelatin-Methacrylate Hydrogels Influences Endothelial Differentiation of Human Mesenchymal Stem Cells.

ICC/IF / Human

Lin Chih-Hsin et al.
J Tissue Eng Regen Med.,  (2018)

publication_link

 

hr_line

Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats.

WB / Rat

Chen Chang-Xing et al.
Oncotarget.,  (2017)

publication_link

 

hr_line