ARG43677

anti-NSE / Neuron Specific Enolase antibody

anti-NSE / Neuron Specific Enolase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes NSE / Neuron Specific Enolase
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NSE / Neuron Specific Enolase
抗原物种 Human
抗原 Synthetic peptide corresponding to Human NSE / Neuron Specific Enolase.
偶联标记 Un-conjugated
Protein Full name Gamma-enolase
別名 Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11

应用说明

应用建议
应用 推荐稀释比
FACS1:50 - 1:100
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 48 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
浓度 Batch dependent
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13807 Mouse ENO2

GeneID: 2026 Human ENO2

GeneID: 24334 Rat ENO2

基因名称 ENO2
全名 enolase 2 (gamma, neuronal)
背景介绍 This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
生物功能 Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt]
细胞定位 Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt]
预测分子量 47 kDa
翻译后修饰 Acetylation; Hydroxylation; Isopeptide bond; Phosphoprotein; Ubl conjugation

检测图片 (5) Click the Picture to Zoom In

  • ARG43677 anti-NSE / Neuron Specific Enolase antibody IHC-P image

    Immunohistochemistry: Human lung cancer stained with ARG43677 anti-NSE / Neuron Specific Enolase antibody.

  • ARG43677 anti-NSE / Neuron Specific Enolase antibody ICC/IF image

    Immunofluorescence: Hela stained with ARG43677 anti-NSE / Neuron Specific Enolase antibody.

  • ARG43677 anti-NSE / Neuron Specific Enolase antibody WB image

    Western blot: Human Fetal Brain stained with ARG43677 anti-NSE / Neuron Specific Enolase antibody.

  • ARG43677 anti-NSE / Neuron Specific Enolase antibody WB image

    Western blot: Rat brain stained with ARG43677 anti-NSE / Neuron Specific Enolase antibody.

  • ARG43677 anti-NSE / Neuron Specific Enolase antibody WB image

    Western blot: Mouse brain stained with ARG43677 anti-NSE / Neuron Specific Enolase antibody.