ARG59461
anti-NLRC4 / CARD12 antibody
anti-NLRC4 / CARD12 antibody for Western blot and Human,Mouse,Rat
NLRC4 Inflammasome Study antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NLRC4 / CARD12 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NLRC4 / CARD12 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 838-874 of Human NLRC4 / CARD12. (KILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDR) |
| 偶联标记 | Un-conjugated |
| 別名 | CLAN; AIFEC; IPAF; CLANA; CARD12; CLANC; CLANB; CLAND; NLR family CARD domain-containing protein 4; Caspase recruitment domain-containing protein 12; Ipaf; CLR2.1; CARD, LRR, and NACHT-containing protein; CLAN1; Ice protease-activating factor; Clan protein; FCAS4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NLRC4 |
| 全名 | NLR family, CARD domain containing 4 |
| 背景介绍 | NLRC4 / CARD12 is a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| 生物功能 | NLRC4 / CARD12 is a key component. of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytosol. Note=Cytoplasmic filaments. [UniProt] |
| 产品亮点 | Related products: NLRC4 antibodies; NLRC4 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | NLRC4 Inflammasome Study antibody |
| 预测分子量 | 116 kDa |
| 翻译后修饰 | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro (By similarity). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59461 anti-NLRC4 / CARD12 antibody WB image
Western blot: 40 µg of 22RV1, 40 µg of RH35 and 40 µg of NIH/3T3 whole cell lysates stained with ARG59461 anti-NLRC4 / CARD12 antibody at 0.5 µg/ml dilution.
