ARG40105

anti-MRPS35 antibody

anti-MRPS35 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes MRPS35
反应物种 Hu, Ms
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MRPS35
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-323 of Human MRPS35 (NP_068593.2).
偶联标记 Un-conjugated
別名 HDCMD11P; S28mt; MRPS28; MRP-S35; MDS023; 28S ribosomal protein S35, mitochondrial; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse kidney and HT-29
实际分子量 ~ 38 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 232536 Mouse MRPS35

GeneID: 60488 Human MRPS35

Swiss-port # P82673 Human 28S ribosomal protein S35, mitochondrial

Swiss-port # Q8BJZ4 Mouse 28S ribosomal protein S35, mitochondrial

基因名称 MRPS35
全名 mitochondrial ribosomal protein S35
背景介绍 Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010]
细胞定位 Mitochondrion. [UniProt]
预测分子量 37 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG40105 anti-MRPS35 antibody WB image

    Western blot: 25 µg of Mouse kidney and HT-29 cell lysates stained with ARG40105 anti-MRPS35 antibody at 1:1000 dilution.