ARG63789
anti-MIF antibody
anti-MIF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes MIF |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MIF |
| 抗原物种 | Human |
| 抗原 | C-NAANVGWNNSTFA |
| 偶联标记 | Un-conjugated |
| 別名 | Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P14174 Human Macrophage migration inhibitory factor |
|---|---|
| 背景介绍 | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 12 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG63789 anti-MIF antibody WB image
Western Blot: Daudi (A) and Jurkat (B) lysate (35 µg protein in RIPA buffer) stained with ARG63789 anti-MIF antibody at 0.01 µg/ml dilution.
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ARG63789 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63789 anti-MIF antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63789 anti-MIF antibody WB image
Western blot: 35 µg of Human thymus lysate (in RIPA buffer) stained with ARG63789 anti-MIF antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
