ARG59873
anti-METTL14 antibody
anti-METTL14 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes METTL14 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | METTL14 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-210 of Human METTL14 (NP_066012.1). |
| 偶联标记 | Un-conjugated |
| 別名 | N6-adenosine-methyltransferase subunit METTL14; EC 2.1.1.62; Methyltransferase-like protein 14 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A549 and Mouse brain | ||||||
| 实际分子量 | 62 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q3UIK4 Mouse N6-adenosine-methyltransferase subunit METTL14 Swiss-port # Q9HCE5 Human N6-adenosine-methyltransferase subunit METTL14 |
|---|---|
| 基因名称 | METTL14 |
| 全名 | methyltransferase like 14 |
| 生物功能 | N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 产品亮点 | Related news: m6A reader YTHDF2 in mRNA decay and aggresome formation; |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylation at Ser-399 is important for interaction with METTL3: phosphorylated Ser-399 forms a salt bridge with 'Arg-471' of METTL3. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59873 anti-METTL14 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue stained with ARG59873 anti-METTL14 antibody at 1:100 dilution.
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ARG59873 anti-METTL14 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG59873 anti-METTL14 antibody at 1:1000 dilution.
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ARG59873 anti-METTL14 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG59873 anti-METTL14 antibody at 1:1000 dilution.
