ARG63069
anti-MAP2ab antibody [MT-08]
anti-MAP2ab antibody [MT-08] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Pig
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MT-08] recognizes MAP2ab |
|---|---|
| 反应物种 | Hu, Ms, Pig |
| 应用 | ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The clone MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MT-08 |
| 同位型 | IgG1 |
| 靶点名称 | MAP2ab |
| 抗原 | Microtubule protein (bovine brain) enriched for kinesin |
| 偶联标记 | Un-conjugated |
| 別名 | MAP2A; Microtubule-associated protein 2; MAP2B; MAP-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| 阳性对照 | WB, IP and ELISA: Porcine brain IHC-P: Brain IHC-Fr: Mouse brain ICC/IF: SH-SY5Y |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MAP2 |
| 全名 | microtubule-associated protein 2 |
| 背景介绍 | MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells. |
| 生物功能 | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt] |
| 产品亮点 | Related products: MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody |
| 预测分子量 | 200 kDa |
| 翻译后修饰 | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG63069 anti-MAP2ab antibody [MT-08] WB image
Western blot: Microtubules partially purified from porcine brain lysate stained with ARG63069 anti-MAP2ab antibody [MT-08].
