ARG40281
anti-Lysozyme antibody
anti-Lysozyme antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Lysozyme |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Lysozyme |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 106-141 of Human Lysozyme. (NIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQ) |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.2.1.17; Lysozyme C; LZM; 1,4-beta-N-acetylmuramidase C |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 19 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LYZ |
| 全名 | lysozyme |
| 背景介绍 | This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014] |
| 生物功能 | Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 17 kDa |
检测图片 (6) Click the Picture to Zoom In
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ARG40281 anti-Lysozyme antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG40281 anti-Lysozyme antibody.
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ARG40281 anti-Lysozyme antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ileum tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40281 anti-Lysozyme antibody (red) at 5 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
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ARG40281 anti-Lysozyme antibody WB image
Western blot: 50 µg of Rat intestine, 50 µg of Rat kidney, 50 µg of Rat liver, 40 µg of HeLa, 40 µg of SW620, 40 µg of 293T and 40 µg of HepG2 whole cell lysates stained with ARG40281 anti-Lysozyme antibody at 0.5 µg/ml dilution.
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ARG40281 anti-Lysozyme antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon organoid tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40281 anti-Lysozyme antibody (red) at 5 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
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ARG40281 anti-Lysozyme antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse ileum tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40281 anti-Lysozyme antibody (red) at 5 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
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ARG40281 anti-Lysozyme antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse ileum organoid tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40281 anti-Lysozyme antibody (red) at 5 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
