ARG65177
anti-LSP1 antibody
anti-LSP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes LSP1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1 |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LSP1 |
| 抗原物种 | Human |
| 抗原 | C-RYKFVATGHGKYEKV |
| 偶联标记 | Un-conjugated |
| 別名 | Lymphocyte-specific protein 1; pp52; 52 kDa phosphoprotein; 47 kDa actin-binding protein; WP34; Lymphocyte-specific antigen WP34 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis (By similarity). |
检测图片 (4) Click the Picture to Zoom In
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ARG65177 anti-LSP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65177 anti-LSP1 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65177 anti-LSP1 antibody WB image
Western blot: 35 µg of Human peripheral blood mononucleocyte (A), lymph node (B) and tonsil (C) lysates (in RIPA buffer) stained with ARG65177 anti-LSP1 antibody at 0.05 µg/ml (A), 0.03 µg/ml (B, C) dilutions and incubated at RT for 1 hour.
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ARG65177 anti-LSP1 antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG65177 anti-LSP1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG65177 anti-LSP1 antibody WB image
Western blot: 35 µg of Mouse spleen lysate (in RIPA buffer) stained with ARG65177 anti-LSP1 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
