ARG63098
anti-LCP2 / SLP76 antibody
anti-LCP2 / SLP76 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LCP2 / SLP76 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LCP2 / SLP76 |
| 抗原物种 | Human |
| 抗原 | Bacterially expressed fusion protein corresponding to aa. 216-434 of Human SLP76 with histidine tag. |
| 偶联标记 | Un-conjugated |
| 別名 | Lymphocyte cytosolic protein 2; SLP-76 tyrosine phosphoprotein; SLP76; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: Jurkat IHC-P: Thymus |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from rabbit serum by precipitation methods. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LCP2 |
| 全名 | lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) |
| 背景介绍 | SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation. |
| 生物功能 | Involved in T-cell antigen receptor mediated signaling. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components. |
检测图片 (1) Click the Picture to Zoom In
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ARG63098 anti-LCP2 / SLP76 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue stained with ARG63098 anti-LCP2 / SLP76 antibody.
