ARG57161
anti-IDH1 antibody [25H10]
anti-IDH1 antibody [25H10] for Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [25H10] recognizes IDH1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 25H10 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | IDH1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-414 of Human IDH1 |
| 偶联标记 | Un-conjugated |
| 別名 | IDPC; EC 1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; IDP; HEL-S-26; HEL-216; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; PICD; IDCD; NADP; Oxalosuccinate decarboxylase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O75874 Human Isocitrate dehydrogenase [NADP] cytoplasmic Swiss-port # O88844 Mouse Isocitrate dehydrogenase [NADP] cytoplasmic |
|---|---|
| 基因名称 | IDH1 |
| 全名 | isocitrate dehydrogenase 1 (NADP+), soluble |
| 背景介绍 | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| 产品亮点 | Related products: Isocitrate Dehydrogenase antibodies; Isocitrate Dehydrogenase ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Acetylation at Lys-374 dramatically reduces catalytic activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG57161 anti-IDH1 antibody [25H10] WB image
Western blot: 40 µg of 1) HepG2, 2) A549, 3) HeLa, 4) NIH/3T3, 5) Jurkat, 6) K562, 7) 293T, 8) MCF7, and 9) LnCaP cell lysates stained with ARG57161 anti-IDH1 antibody [25H10] at 1:1000 dilution.
