ARG65636

anti-HMGB1 antibody

anti-HMGB1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Gene Regulation antibody
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anti-HMGB1 antibody

Rabbit Polyclonal antibody recognizes HMGB1

ARG65636 Arigo biolaboratories
4.7 out of 5 based on 3
anti-HMGB1 antibody is rated 4.7 out of 5 by 3.

anti-HMGB1 antibody

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Overview

Product Description

Rabbit Polyclonal antibody recognizes HMGB1

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGB1
Antigen Species Human
Immunogen Synthetic peptide of human HMGB1
Conjugation Un-conjugated
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Application Suggestion
Application Dilution
IHC-P50-200
WB500-2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 1.8 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 15289 Mouse HMGB1

GeneID: 3146 Human HMGB1

Swiss-port # P09429 Human High mobility group protein B1

Swiss-port # P63158 Mouse High mobility group protein B1

Gene Symbol HMGB1
Gene Full Name high mobility group box 1
Background High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
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Research Area Gene Regulation antibody
PTM Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).

Images (4) Click the Picture to Zoom In

  • ARG65636 anti-HMGB1 antibody WB image

    Western blot: 30 µg of Jurkat, HeLa, HUVEC, Mouse spleen, Mouse lung, Mouse liver, Rat spleen, and Rat lung lysates stained with ARG65636 anti-HMGB1 antibody at 1:200 dilution.

  • ARG65636 anti-HMGB1 antibody WB image

    Western blot: 20 µg of HeLa, Mouse lung, and Rat lung lysates stained with ARG65636 anti-HMGB1 antibody at 1:500 dilution.

  • ARG65636 anti-HMGB1 antibody image

    Immunohistochemistry: paraffin-embedded Human thyroid cancer stained with ARG65636 anti-HMGB1 antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)

  • ARG65636 anti-HMGB1 antibody WB image

    Western blot: 20 µg of Huh7, Huh7 + shHMGB1 and 20 ng of rHMGB1, rHMGB2 stained with ARG65636 anti-HMGB1 antibody at 1:2000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-HMGB1 antibody

Application:WB

Sample:Huh7 and Huh7 shHMGB1

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-HMGB1 antibody

Application:WB

Sample:HeLa, Mouse lung and Rat lung

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Good

Review for anti-HMGB1 antibody

Application:WB

Sample:THP-1

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Specific References

Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.

IHC-P / Mouse

Chuang MH et al.
Integr Cancer Ther., 1 (2016)

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