ARG65636

anti-HMGB1 antibody

anti-HMGB1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Gene Regulation antibody
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anti-HMGB1 antibody

Rabbit Polyclonal antibody recognizes HMGB1

ARG65636 Arigo biolaboratories
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anti-HMGB1 antibody is rated 4.7 out of 5 by 3.

anti-HMGB1 antibody

publication_link 参考文献1

概述

产品描述

Rabbit Polyclonal antibody recognizes HMGB1

反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 HMGB1
抗原物种 Human
抗原 Synthetic peptide of human HMGB1
偶联标记 Un-conjugated
全名 high mobility group box 1
別名 HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

应用说明

应用建议
应用 推荐稀释比
IHC-P50-200
WB500-2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 25 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol
抗菌剂 0.05% Sodium azide
稳定剂 40% Glycerol
浓度 1.8 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 15289 Mouse HMGB1

GeneID: 3146 Human HMGB1

Swiss-port # P09429 Human High mobility group protein B1

Swiss-port # P63158 Mouse High mobility group protein B1

基因名称 HMGB1
基因全名 high mobility group box 1
背景介绍 HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
生物功能 HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
产品亮点 Related products:
HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 ELISA Kit for your research
Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit
New HMGB1 neutralizing antibody is released
Related poster download:
HMGB1 Pathway.pdf
研究领域 Gene Regulation antibody
翻译后修饰 Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).

检测图片 (4) Click the Picture to Zoom In

  • ARG65636 anti-HMGB1 antibody WB image

    Western blot: 30 µg of Jurkat, HeLa, HUVEC, Mouse spleen, Mouse lung, Mouse liver, Rat spleen, and Rat lung lysates stained with ARG65636 anti-HMGB1 antibody at 1:200 dilution.

  • ARG65636 anti-HMGB1 antibody WB image

    Western blot: 20 µg of HeLa, Mouse lung, and Rat lung lysates stained with ARG65636 anti-HMGB1 antibody at 1:500 dilution.

  • ARG65636 anti-HMGB1 antibody image

    Immunohistochemistry: paraffin-embedded Human thyroid cancer stained with ARG65636 anti-HMGB1 antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)

  • ARG65636 anti-HMGB1 antibody WB image

    Western blot: 20 µg of Huh7, Huh7 + shHMGB1 and 20 ng of rHMGB1, rHMGB2 stained with ARG65636 anti-HMGB1 antibody at 1:2000 dilution.

客户反馈

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anti-HMGB1 antibody

Application:WB

Sample:Huh7 and Huh7 shHMGB1

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-HMGB1 antibody

Application:WB

Sample:HeLa, Mouse lung and Rat lung

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-HMGB1 antibody

Application:WB

Sample:THP-1

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.

IHC-P / Mouse

Chuang MH et al.
Integr Cancer Ther., 1 (2016)

publication_link

 

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