ARG42193
anti-HLA A antibody
anti-HLA A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HLA A |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HLA A |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 82-106 of Human HLA A. (EYWDGETRKVKAHSQTHRVDLGTLR) |
| 偶联标记 | Un-conjugated |
| 別名 | MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | U87 and HepG2 | ||||||||
| 实际分子量 | ~ 41 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P04439 Human HLA class I histocompatibility antigen, A alpha chain |
|---|---|
| 基因名称 | HLA-A |
| 全名 | major histocompatibility complex, class I, A |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG42193 anti-HLA A antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG42193 anti-HLA A antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42193 anti-HLA A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue stained with ARG42193 anti-HLA A antibody.
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ARG42193 anti-HLA A antibody WB image
Western blot: 40 µg of U87 and HepG2 whole cell lysates stained with ARG42193 anti-HLA A antibody at 0.5 µg/ml dilution.
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ARG42193 anti-HLA A antibody WB image
Western blot: 50 µg of sample under reducing conditions. U-87MG and HL-60 whole cell lysates stained with ARG42193 anti-HLA A antibody at 0.5 µg/ml dilution, overnight at 4°C.
