ARG22247
anti-GluR1 antibody [S355-1]
anti-GluR1 antibody [S355-1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
概述
| 产品描述 | Mouse Monoclonal antibody [S355-1] recognizes GluR1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects ~100kDa. Does not cross-react with GluR2. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S355-1 |
| 同位型 | IgG1 |
| 靶点名称 | GluR1 |
| 抗原物种 | Rat |
| 抗原 | Fusion protein around aa. 1-389 (extracellular N-terminus) of Rat GluR1 |
| 偶联标记 | Un-conjugated |
| 別名 | GLUH1; GluA1; GluR-1; Glutamate receptor ionotropic, AMPA 1; GluR-K1; GLUR1; HBGR1; AMPA-selective glutamate receptor 1; GluR-A; GLURA; Glutamate receptor 1 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.1% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Gria1 |
| 全名 | glutamate receptor, ionotropic, AMPA 1 |
| 背景介绍 | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. [UniProt] |
| 细胞定位 | Cell Junction, Cell membrane, Endoplasmic Reticulum |
| 产品亮点 | Related products: GluR1 antibodies; GluR1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Viral-like capsids, new trans-synaptic mRNA transport mechanism |
| 研究领域 | Neuroscience antibody; Postsynaptic Receptor antibody |
| 预测分子量 | 102 kDa |
| 翻译后修饰 | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2. |
检测图片 (2) Click the Picture to Zoom In
-
ARG22247 anti-GluR1 antibody [S355-1] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22247 anti-GluR1 antibody [S355-1] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22247 anti-GluR1 antibody [S355-1] (D) Composite.
-
ARG22247 anti-GluR1 antibody [S355-1] WB image
Western blot: 1) MW ladder, 2) 10 µg of Rat brain membrane lysates stained with ARG22247 anti-GluR1 antibody [S355-1] at 1:2000 dilution (60 min at RT). Block: 5% milk + TBST O/N at 4°C.
