anti-GSDMD antibody

anti-GSDMD antibody for ICC/IF,Western blot and Human,Mouse,Rat

Pyroptosis Study antibody
publication_link 参考文献4


产品描述 Rabbit Polyclonal antibody recognizes GSDMD
反应物种 Hu, Ms, Rat
特异性 The antibody reacts to full length and N-terminal fragment (1-275) of GSDMD protein.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GSDMD
抗原物种 Mouse
抗原 Recombinant fusion protein within aa. 1-275 of GSDMD.
偶联标记 Un-conjugated
別名 FKSG10; DF5L; Gasdermin domain-containing protein 1; Gasdermin-D; DFNA5L; GSDMDC1


应用 推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse lung
实际分子量 ~ 53 kDa


形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 69146 Mouse GSDMD

GeneID: 79792 Human GSDMD

Swiss-port # P57764 Human Gasdermin-D

Swiss-port # Q9D8T2 Mouse Gasdermin-D

基因名称 GSDMD
全名 gasdermin D
背景介绍 GSDMD (Gasdermin D) is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
生物功能 GSDMD, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes. Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216). [UniProt]
细胞定位 Gasdermin-D: Cytoplasm, cytosol. Inflammasome. Note=In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. Gasdermin-D, N-terminal: Cell membrane. Secreted. Note=Released in the extracellular milieu following pyroptosis. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
Related products:
GSDMD antibodies; GSDMD Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
Solutions for studying PANoptosis & PANoptosome
研究领域 Pyroptosis Study antibody
预测分子量 53 kDa
翻译后修饰 Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms) or CASP4 relieves autoinhibition and is sufficient to initiate pyroptosis. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG41404 anti-GSDMD antibody ICC/IF image

    Immunofluorescence: RAW264.7 cells stained with ARG41404 anti-GSDMD antibody at 1:100 dilution.

  • ARG41404 anti-GSDMD antibody WB image

    Western blot: 25 µg of Mouse lung lysate stained with ARG41404 anti-GSDMD antibody at 1:1000 dilution.


Elaidic acid induced hepatocyte pyroptosis via autophagy-CTSB-NLRP3 pathway

WB / Mouse

Jing Lu et al.
Food Chem Toxicol.,  (2023)




Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2‑induced pyroptosis

WB / Human

Yulin Yuan et al.
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Curcumin Ameliorates White Matter Injury after Ischemic Stroke by Inhibiting Microglia/Macrophage Pyroptosis through NF- κ B Suppression and NLRP3 Inflammasome Inhibition

WB / Mouse

Yuanyuan Ran et al.
Oxid Med Cell Longev.,  (2021)




Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)