ARG10122

anti-GFAP antibody [GF5]

anti-GFAP antibody [GF5] for ELISA,ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
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publication_link 参考文献4

概述

产品描述

Mouse Monoclonal antibody [GF5] recognizes Glial Fibrillary Acidic Protein (GFAP)

反应物种 Hu, Ms, Rat
应用 ELISA, ICC/IF, IHC-Fr, WB
特异性 This antibody is specific for Human GFAP. There is no cross-reactivity with other neurospecific proteins.
宿主 Mouse
克隆 Monoclonal
克隆号 GF5
同位型 IgG2b
靶点名称 GFAP
抗原物种 Human
抗原 43-45 kD band corresponding to GFAP in immunoblotting of extract from human brain and spinal cord.
偶联标记 Un-conjugated
別名 Glial fibrillary acidic protein; ALXDRD; GFAP

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
ICC/IF1:100 - 1:1000
IHC-Fr1:100 - 1:1000
WB1:250 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Protein G affinity purified.
缓冲液 PBS (pH 7.4) and 0.1% Sodium azide
抗菌剂 0.1% Sodium azide
浓度 1.0-2.0 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

基因名称 GFAP
全名 glial fibrillary acidic protein
背景介绍 GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
生物功能 GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30304 Astrocyte Maturation / Muller Cell Marker Antibody Duo (GFAP, Vimentin)
ARG30315 Brain Injury IHC Marker Antibody Duo (GFAP, MMP9)
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Microglial help TAM-ing inflammation in the brain
Astrocyte-to-neuron conversion for Parkinson's disease treatment
研究领域 Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
预测分子量 50 kDa
翻译后修饰 Phosphorylated by PKN1.

检测图片 (3) Click the Picture to Zoom In

  • ARG10122 anti-GFAP antibody [GF5] ICC/IF image

    Immunofluorescence: Rat astrocyte primary cell stained with ARG10122 anti-GFAP antibody [GF5] (green) at 1:200 dilution.
    Cell nuclei was stained with DAPI (blue).

  • ARG10122 anti-GFAP antibody [GF5] WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.

  • ARG10122 anti-GFAP antibody [GF5] WB image

    Western blot: 30 µg of Rat brain lysate stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.

客户反馈

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anti-GFAP antibody [GF5]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-GFAP antibody [GF5]

Application:IF/ICC

Sample:Rat astrocyte primary cell

Fixation Buffer:PFA

Primary Antibody Dilution Factor:1:200

文献引用

Hyperbaric Oxygen Therapy Attenuates Burn-Induced Denervated Muscle Atrophy

IHC-Fr / Rat

Chin-An Chen et al.
Int J Med Sci.,  (2021)

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RIP3/MLKL pathway-regulated necroptosis: A new mechanism of paclitaxel-induced peripheral neuropathy

IHC-Fr / Rat

Dongyang Ma et al.
J Biochem Mol Toxicol.,  (2021)

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Mechanisms of bupivacaine inhibition of glutamate-induced intracellular calcium elevation in primary cultured rat hippocampal astrocytes.

ICC/IF / Rat

Huan-huan Ma et al.
药学学报,  (2020)

publication_link

 

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Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain Hypersensitivity in a Rat Model of Inflammatory Pain.

WB, IF, IHC-Fr / Rat

Luo Zhenzhao et al.
Cell Mol Neurobiol.,  (2019)

publication_link

 

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