ARG62992
anti-GFAP antibody [GF-02]
anti-GFAP antibody [GF-02] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Pig
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [GF-02] recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| 反应物种 | Hu, Pig |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | The clone GF-02 exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GF-02 |
| 同位型 | IgM |
| 靶点名称 | GFAP |
| 抗原物种 | Pig |
| 抗原 | Pellet of porcine brain cold-stable proteins after depolymerization of microtubules. |
| 偶联标记 | Un-conjugated |
| 別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by thiophilic adsorption-affinity chromatography and precipitation methods. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | TBS (pH 8.0) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 背景介绍 | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 生物功能 | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| 产品亮点 | Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgM secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Phosphorylated by PKN1. |
检测图片 (1) Click the Picture to Zoom In
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ARG62992 anti-GFAP antibody [GF-02] WB image
Western blot: Porcine brain lysate stained with ARG62992 anti-GFAP antibody [GF-02].
Lane 1: immunostaining with anti-GFAP antibody. Lane 2: immunostaining with Isotype mouse IgM control.
