ARG55954
anti-GFAP antibody [GA-5]
anti-GFAP antibody [GA-5] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [GA-5] recognizes GFAP |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GA-5 |
| 同位型 | IgG1 |
| 靶点名称 | GFAP |
| 抗原物种 | Pig |
| 抗原 | GFAP isolated from Pig spinal cord. |
| 偶联标记 | Un-conjugated |
| 別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GFAP |
| 全名 | glial fibrillary acidic protein |
| 背景介绍 | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 生物功能 | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 产品亮点 | Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Phosphorylated by PKN1. |
检测图片 (3) Click the Picture to Zoom In
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ARG55954 anti-GFAP antibody [GA-5] WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG55954 anti-GFAP antibody [GA-5] at 1:1000 dilution.
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ARG55954 anti-GFAP antibody [GA-5] IHC-P image
Immunohistochemistry: Human brain stained with ARG55954 anti-GFAP antibody [GA-5]. Note cytoplasmic staining.
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ARG55954 anti-GFAP antibody [GA-5] WB image
Western blot: Human brain lysate stained with ARG55954 anti-GFAP antibody [GA-5].
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anti-GFAP antibody [GA-5]
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
