ARG52312

anti-GFAP antibody

anti-GFAP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
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publication_link 参考文献3

概述

产品描述

Rabbit Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)

反应物种 Ms, Rat
应用 ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GFAP
抗原物种 Bovine
抗原 Recombinant and purified bovine GFAP
偶联标记 Un-conjugated
別名 Glial fibrillary acidic protein; ALXDRD; GFAP

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:1,000
IHC-FoFr Assay-dependent
IHC-Fr1:500 - 1:2000
IHC-P1:500 - 1:2000
WB1:5,000
应用说明 Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Neat Serum
缓冲液 Neat serum
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

Swiss-port # P03995 Mouse Glial fibrillary acidic protein

Swiss-port # P47819 Rat Glial fibrillary acidic protein

基因名称 GFAP
全名 glial fibrillary acidic protein
背景介绍 GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
生物功能 GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin)
ARG30007 Astrocyte Marker Antibody Duo (Host: Goat, Rabbit)
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Microglial help TAM-ing inflammation in the brain
Astrocyte-to-neuron conversion for Parkinson's disease treatment
研究领域 Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
预测分子量 50 kDa
翻译后修饰 Phosphorylated by PKN1.

检测图片 (3) Click the Picture to Zoom In

  • ARG52312 anti-GFAP antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse C57BL/6Jnarl brain tissue. The tissue section was fixed by 4% formalin and blocked with BSA with 3% Goat serum, at RT for 1 hour. Tissue section was then stained with ARG52312 anti-GFAP antibody at 1:500 dilution, in PBS with 1% Goat serum, overnight at 4°C.

    Blue: DAPI
    Yellow: Venus reporter gene
    Red: GFAP

  • ARG52312 anti-GFAP antibody ICC/IF image

    Immunofluorescence: Cultured neurons and glia stained with ARG52312 anti-GFAP antibody (red) and ARG52468 anti-Vimentin antibody (green) showing specific labeling of GFAP (red) and vimentin (green). Cells containing GFAP and vimentin appear yellow

  • ARG52312 anti-GFAP antibody WB image

    Western blot: Rat cortex lysate showing specific immunolabeling of the ~50 kDa GFAP protein stained with ARG52312 anti-GFAP antibody.

客户反馈

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anti-GFAP antibody

Application:IHC

Sample:Mouse C57BL/6Jnarl brain tissue

Sample Preparation Method:Frozen

Fixation Buffer:4% formalin

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:Dylight 594

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anti-GFAP antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Nose to brain delivery of Astragaloside IV by β-Asarone modified chitosan nanoparticles for multiple sclerosis therapy

IHC-P / Mouse

Xiao Zhao et al.
Int J Pharm.,  (2023)

publication_link

 

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Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia

ICC/IF / Mouse

Miao Tang et al.
Pharm Biol.,  (2022)

publication_link

 

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Toll-Like Receptor 4 Mediates Methamphetamine-Induced Neuroinflammation through Caspase-11 Signaling Pathway in Astrocytes

IHC-P, IHC-Fr / Mouse

Si-Hao Du et al.
Front Mol Neurosci.,  (2017)

publication_link

 

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