ARG52312
anti-GFAP antibody
anti-GFAP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
参考文献3
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GFAP |
| 抗原物种 | Bovine |
| 抗原 | Recombinant and purified bovine GFAP |
| 偶联标记 | Un-conjugated |
| 別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Neat Serum |
| 缓冲液 | Neat serum |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GFAP |
| 全名 | glial fibrillary acidic protein |
| 背景介绍 | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 生物功能 | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin) ARG30007 Astrocyte Marker Antibody Duo (Host: Goat, Rabbit) Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Microglial help TAM-ing inflammation in the brain Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Phosphorylated by PKN1. |
检测图片 (4) Click the Picture to Zoom In
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ARG52312 anti-GFAP antibody IHC-Fr image
Immunohistochemistry: Frozen Mouse hippocampus. stained with ARG52312 anti-GFAP antibody.
From Tang M et al. Pharm Biol. (2022), doi: 10.1080/13880209.2022.2108064, Fig. 4. A.
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ARG52312 anti-GFAP antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse C57BL/6Jnarl brain tissue. The tissue section was fixed by 4% formalin and blocked with BSA with 3% Goat serum, at RT for 1 hour. Tissue section was then stained with ARG52312 anti-GFAP antibody at 1:500 dilution, in PBS with 1% Goat serum, overnight at 4°C.
Blue: DAPI
Yellow: Venus reporter gene
Red: GFAP -
ARG52312 anti-GFAP antibody ICC/IF image
Immunofluorescence: Cultured neurons and glia stained with ARG52312 anti-GFAP antibody (red) and ARG52468 anti-Vimentin antibody (green) showing specific labeling of GFAP (red) and vimentin (green). Cells containing GFAP and vimentin appear yellow
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ARG52312 anti-GFAP antibody WB image
Western blot: Rat cortex lysate showing specific immunolabeling of the ~50 kDa GFAP protein stained with ARG52312 anti-GFAP antibody.
客户反馈
Good
anti-GFAP antibody
Application：IHC
Sample：Mouse C57BL/6Jnarl brain tissue
Sample Preparation Method：Frozen
Fixation Buffer：4% formalin
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：Dylight 594
Good
anti-GFAP antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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