ARG10716
anti-GAP43 antibody [5E8]
anti-GAP43 antibody [5E8] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [5E8] recognizes GAP43 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5E8 |
| 同位型 | IgG1 |
| 靶点名称 | GAP43 |
| 抗原物种 | Human |
| 抗原 | Recombinant full-length Human GAP43. |
| 偶联标记 | Un-conjugated |
| 別名 | pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GAP43 |
| 全名 | growth associated protein 43 |
| 背景介绍 | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
检测图片 (4) Click the Picture to Zoom In
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ARG10716 anti-GAP43 antibody [5E8] ICC/IF image
Immunocytochemistry: Mixed Rat neuronal cultures stained with ARG10716 anti-GAP43 antibody [5E8] (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.
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ARG10716 anti-GAP43 antibody [5E8] ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10716 anti-GAP43 antibody [5E8] (red) at 1:1000 dilution and costained with ARG52313 anti-GFAP antibody (green) at 1:2000 dilution. DAPI (blue) for nuclear staining.
The GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while the GFAP antibody stains astroglial cells.
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ARG10716 anti-GAP43 antibody [5E8] WB image
Western blot: 1) SH-SY5Y cell lysate, and 2) whole Rat brain lysate was stained with ARG10716 anti-GAP43 antibody [5E8] at 1: 2000 dilution.
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ARG10716 anti-GAP43 antibody [5E8] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, C6 and SH-SY5Y cell lysates stained with ARG10716 anti-GAP43 antibody [5E8] (green) at 1:2000 dilution.
The protein is expressed in rodent and Human neurons and neuronal derived cells but not in C6 cells which are of glial origin.
