ARG40713

anti-FOXG1 antibody

anti-FOXG1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes FOXG1
反应物种 Hu, Ms
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 FOXG1
抗原物种 Human
抗原 KLH-conjugated synthetic peptide between aa. 225-252 of Human FOXG1.
偶联标记 Un-conjugated
別名 hBF-2; QIN; BF-2; FOXG1C; FOXG1B; FOXG1A; BF-1; HBF2; BF2; BF1; Forkhead box protein G1; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; FKHL2; FKHL3; Forkhead-related protein FKHL2; FKHL1; FKHL4; FKH2; HFK3; HFK2; HFK1; KHL2; Forkhead-related protein FKHL1; Forkhead-related protein FKHL3; HBF-2; HBF-3; HBF-1; Brain factor 2; Brain factor 1; HBF-G2; FHKL3

应用说明

应用建议
应用 推荐稀释比
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 52 kDa

属性

形式 Liquid
纯化 Purification with Protein A and immunogen peptide.
缓冲液 PBS and 0.09% (W/V) Sodium azide.
抗菌剂 0.09% (W/V) Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 15228 Mouse FOXG1

GeneID: 2290 Human FOXG1

Swiss-port # P55316 Human Forkhead box protein G1

Swiss-port # Q60987 Mouse Forkhead box protein G1

基因名称 FOXG1
全名 forkhead box G1
背景介绍 This locus encodes a member of the forked-head transcription factor family. The encoded protein, which functions as a repressor, may play a role in brain development. Mutations at this locus have been associated with Rett syndrome. [provided by RefSeq, Feb 2012]
生物功能 Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 52 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG40713 anti-FOXG1 antibody ICC/IF image

    Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG40713 anti-FOXG1 antibody (green) at 1:25, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated Phalloidin (red). Nuclei were counterstained with DAPI (blue).

  • ARG40713 anti-FOXG1 antibody FACS image

    Flow Cytometry: U251 cells stained with ARG40713 anti-FOXG1 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.

  • ARG40713 anti-FOXG1 antibody WB image

    Western blot: 20 µg of Human brain lysate stained with ARG40713 anti-FOXG1 antibody at 1:1000 dilution.

  • ARG40713 anti-FOXG1 antibody WB image

    Western blot: 35 µg of Mouse brain lysate stained with ARG40713 anti-FOXG1 antibody.