ARG63968
anti-Ezrin antibody
anti-Ezrin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Ezrin |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Ezrin |
| 抗原物种 | Human |
| 抗原 | C-QDYEEKTKKAER |
| 偶联标记 | Un-conjugated |
| 別名 | Ezrin; p81; HEL-S-105; VIL2; CVL; Cytovillin; CVIL; Villin-2 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30231 Phospho Ezrin Antibody Duo (Total, pT567) Related products: Ezrin antibodies; Ezrin Duos / Panels; Anti-Goat IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
检测图片 (3) Click the Picture to Zoom In
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ARG63968 anti-Ezrin antibody WB image
Western blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG63968 anti-Ezrin antibody at 1 µg/ml dilution.
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ARG63968 anti-Ezrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63968 anti-Ezrin antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63968 anti-Ezrin antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63968 anti-Ezrin antibody at 3.8 µg/ml dilution followed by AP-staining.
