ARG58589
anti-Endothelin B Receptor antibody
anti-Endothelin B Receptor antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Endothelin B Receptor |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Endothelin B Receptor |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 406-439 of Human Endothelin B Receptor. (QSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKY) |
| 偶联标记 | Un-conjugated |
| 別名 | HSCR; WS4A; HSCR2; ETB1; Endothelin receptor non-selective type; ETRB; ETB; ET-B; ET-BR; Endothelin B receptor; ABCDS; ETBR |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EDNRB |
| 全名 | endothelin receptor type B |
| 背景介绍 | The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| 生物功能 | Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG58589 anti-Endothelin B Receptor antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG58589 anti-Endothelin B Receptor antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG58589 anti-Endothelin B Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse Testis Tissue stained with ARG58589 anti-Endothelin B Receptor antibody.
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ARG58589 anti-Endothelin B Receptor antibody WB image
Western blot: 40 µg of HeLa whole cell lysate stained with ARG58589 anti-Endothelin B Receptor antibody at 0.5 µg/ml dilution.
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ARG58589 anti-Endothelin B Receptor antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG58589 anti-Endothelin B Receptor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58589 anti-Endothelin B Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat Testis Tissue stained with ARG58589 anti-Endothelin B Receptor antibody.
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ARG58589 anti-Endothelin B Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human Placenta Tissue stained with ARG58589 anti-Endothelin B Receptor antibody.
