ARG45248

anti-EPLIN antibody

anti-EPLIN antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Polyclonal antibody recognizes EPLIN
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 EPLIN
抗原物种 Human
抗原 Recombinant protein containing to human EPLIN.
偶联标记 Un-conjugated
別名 LIMA1; LIM Domain And Actin Binding 1; EPLIN; Epithelial Protein Lost In Neoplasm Beta; LIM Domain And Actin-Binding Protein 1; SREBP3; Sterol Regulatory Element Binding Protein 3; Epithelial Protein Lost In Neoplasm; LDLCQ8

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 85 kDa; 100 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 51474 Human LIMA1

Swiss-port # Q9UHB6 Human LIM domain and actin-binding protein 1

基因名称 LIMA1
全名 LIM Domain And Actin Binding 1
背景介绍 This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011].
生物功能 Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. [UniProt]
细胞定位 Cell junction ; Cell membrane ; Cell projection ; Cytoplasm ; Cytoskeleton ; Membrane. [UniProt]
预测分子量 85 kDa
翻译后修饰 Acetylation ; Phosphoprotein. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45248 anti-EPLIN antibody IHC-P image

    Immunohistochemistry: Human lung squamous cell carcinoma stained with ARG45248 anti-EPLIN antibody at 2 μg/ml dilution.

  • ARG45248 anti-EPLIN antibody ICC/IF image

    Immunofluorescence: U20S stained with ARG45248 anti-EPLIN antibody at 5 ug/ml dilution.

  • ARG45248 anti-EPLIN antibody WB image

    Western blot: HepG2 and T-47D stained with ARG45248 anti-EPLIN antibody at 0.5 μg/ml dilution.

  • ARG45248 anti-EPLIN antibody FACS image

    Flow Cytometry: THP-1 stained with ARG45248 anti-EPLIN antibody at 1 µg/10^6 cells dilution.